Single-use media preparation

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Measuring point	Product	Alternatives
Component A Feed	Proline Promass U 500 Coriolis flowmeter
Flow Component B Feed	Proline Promass U 500 Coriolis flowmeter
WFI Feed	Proline Promass U 500 Coriolis flowmeter
Media Temperature Measurement	iTHERM CompactLine TM311 Compact thermometer	iTHERM CableLine TST310 Cable probe
Media Conductivite Measurement	Digital 4-electrode conductivity sensor Memosens CLS82E
Raman Analyzer	Raman optic system for single use
Media pH Measurement	Digital pH sensor Memosens CPS61E	Digital non-glass pH sensor Memosens CPS77E

Flow measurement in: Component A Feed

Endress+Hauser offers a suite of analytical instruments ideal for this stage.

Product picture of Proline Promass U 500

Proline Promass U 500 Coriolis flowmeter

First single-use flowmeter for biotech applications with premium performance, cGMP compliance, and up to 4 I/Os

General Requirements:

High reproducibility over a large flow range
Suitable for measuring WFI (water for injection)
Measure changing fluids w/o readjustment
Real time density measurement with high accuracy

Technical requirements:

Measuring task: Liquids
Process temperature: max 40°C (104°F)
Density: 0.9 to 1.5 g/cm³

Flow measurement in: Flow Component B Feed

Endress+Hauser offers a suite of analytical instruments ideal for this stage.

Proline Promass U 500 Coriolis flowmeter

First single-use flowmeter for biotech applications with premium performance, cGMP compliance, and up to 4 I/Os

General Requirements:

High reproducibility over a large flow range
Suitable for measuring WFI (water for injection)
Measure changing fluids w/o readjustment
Real time density measurement with high accuracy

Technical requirements:

Measuring task: Liquids
Process temperature: max 40°C (104°F)
Density: 0.9 to 1.5 g/cm³

measurement in: WFI Feed

Endress+Hauser offers a suite of analytical instruments ideal for this stage.

Proline Promass U 500 Coriolis flowmeter

First single-use flowmeter for biotech applications with premium performance, cGMP compliance, and up to 4 I/Os

General Requirements:

High reproducibility over a large flow range
Suitable for measuring WFI (water for injection)

Technical requirements:

Measuring task: Liquids
Process temperature: max 40°C (104°F)
Density: 0.9 to 1.5 g/cm³

Temperature measurement in: Media Temperature Measurement

Endress+Hauser offers a suite of analytical instruments ideal for this stage.

Pt100 temperature sensor - iTHERM CompactLine TM311

iTHERM CompactLine TM311 Compact thermometer

Metric/imperial RTD 4-20 mA/IO-Link compact thermometer for industrial and hygienic applications

Alternatives:

Pt100 RTD thermometer TST310 temperature cable probe

iTHERM CableLine TST310 Cable probe

Metric RTD thermometer with connecting cable for use in many process and laboratory applications

General Requirements:

High accuracy
Wide measurement range
Fast response time

Technical requirements:

Measuring task: Thermometer
Process temperature: max 40°C (104°F)
Density: 0.9 to 1.5 g/cm³

Analysis measurement in: Media Conductivite Measurement

Endress+Hauser offers a suite of analytical instruments ideal for this stage.

Memosens CLS82E - Hygienic 4-electrode conductivity sensor for life sciences, food & beverage

Digital 4-electrode conductivity sensor Memosens CLS82E

Memosens 2.0 contacting conductivity sensor for hygienic applications in life sciences, food & bev

General Requirements:

High accuracy
Wide measurement range
Fast response time

Technical requirements:

Measuring task: Conductivity
Process temperature: max 40°C (104°F)
Density: 0.9 to 1.5 g/cm³

Analysis measurement in: Raman Analyzer

Endress+Hauser offers a suite of analytical instruments ideal for this stage.

Raman optic system for single use

Ensure sterility and reliability for single-use biopharmaceutical development and manufacturing

General Requirements:

Multiparameter measurement
Non-destructive
Rapid quality evaluation
Suitable for analysis of solids and liquids

Technical requirements:

Analysis measurement in: Media pH Measurement

Endress+Hauser offers a suite of analytical instruments ideal for this stage.

Memosens CPS61E - Digital pH sensor for the food industry and bioreactors in the biotech industry

Digital pH sensor Memosens CPS61E

Memosens 2.0 glass sensor for the food & beverage industry and for bioreactors in biotech industry

Alternatives:

Memosens CPS77E - ISFET pH sensor for food & beverage and life sciences industries

Digital non-glass pH sensor Memosens CPS77E

Memosens 2.0 ISFET pH electrode for food & beverage and life sciences industries

General Requirements:

High accuracy
Easy and accurate calibration

Technical requirements:

Measuring task: pH
Process temperature: max 40°C (104°F)
Density: 0.9 to 1.5 g/cm³

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Key instrumentation challenges in single-use media preparation

In single-use media preparation the biggest challenges are usually no the mixing bag itself, but the quality and reliability of the measurements use to verify media preparation. Media preparation remains one of the least-measured unit operations in single-use bioprocessing.

Obtaining reliable pH measurements in disposable systems: Disposable pH sensors can exhibit drift, limited calibration capability and lower reliability compared with conventional reusable probes.
Verifying complete powder dissolution and mixing homogeneity: Instrumentation often cannot directly confirm that all powder is dissolved and uniformly distributed.
Accurate bag volume/level measurement: Flexible bags change shape during filling and emptying, making precise level measurement difficult.
Maintaining data integrity with single-use sensors: Disposable instrumentation must still meet GMP data requirements while integrating into plant automation systems.

Media preparation portfolio highlights

Explore our innovations for your single-use media preparation process

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